Clonal Micropropagation of Gentiana scabra BUNGE var. buergeri MAXIM. and Examination of the Homogeneity Concerning the Gentiopicroside Content

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When the axillary buds of Gentiana scabra BUNGE var. buergeri MAXIM. were cultured on Murashige-Skoog (MS)medium supplemented with a combination of gibberellin A_3 (GA) and 6-benzylaminopurine (BAP) (1mg/l each) at 25℃ under 16h light conditions for 60d, the direct multiple shoot formation (18.5 shoots per axillary bud) occurred. The MS medium containing Ga-BAP (2.5mg/l each) mostly stimulated the multiple shoot formation having approximately 71 shoots per tissue in the second subculture of the regenerated shoot. The hormone free MS medium stimulated root formation. These clonally propagated plants were transplanted to vermiculite. Quantitative analysis of gentiopicroside contained in the root of plantlets was carried out by high performance liquid chromatography using p-anisic acid as an internal standard. Obviously, the variation of the clonally propagated plants was smaller than that of the cultivated plants bred by morphological selection. From this system, 4.7×10^8 clonally propagated plants can be theoretically obtained from an axillary bud in a year.
1991-01-25