Induction of Apoptosis in Cultured Rat Gastric Epithelial Cells by Ipriflavone : Comparison with Indomethacin (Pharmacology)
スポンサーリンク
概要
- 論文の詳細を見る
Based on a previous report illustrating severe gastrointestinal side effects with ipriflavone, we examined the effects of ipriflavone on cell death in cultured rat gastric epithelial cells compared with other chemicals, including indomethacin. Low concentrations of ipriflavone, indomethacin, dexamethasone, and estradiol all induced cell death in gastric epithelial cells. DNA from cultured cells treated with ipriflavone showed fragmentation by electrophoresis. Also, some of the cultured cells treated with ipriflavone were positively stained by TUNEL. In order to confirm induction of apoptosis by ipriflavone, rescue from ipriflavone-induced cell death with Z-DEVD-FMK (0.02-0.1 mM), which is a caspase 3 inhibitor, or PGE_2 (0.01-10 mM), was tested. Only Z-DEVD-FMK was observed to rescue the cells from cell death. Similar results were obtained with indomethacin, dexamethasone and estradiol. These results suggest that ipriflavone, indomethacin, dexamethasone and estradiol induce cell death of cultured rat gastric epithelial cells by apoptosis.
- 社団法人日本薬学会の論文
- 2004-05-01
著者
-
TANI Satoru
Faculty of Pharmaceutical Sciences Josai University
-
TANAKA Toru
Faculty of Pharmaceutical Sciences Josai University
-
Tani Satoru
Faculty Of Pharmaceutical Sciences Josai University:life Science Research Center Josai University
-
Tani Satoru
Faculty Of Phamaceutical Sciences Josai University:life Science Research Center Josai University
-
MATSUDA Kazuki
Faculty of Pharmaceutical Sciences, Josai University
-
Matsuda Kazuki
Faculty Of Pharmaceutical Sciences Josai University
-
Tanaka Toru
Faculty Of Phamaceutical Sciences Josai University
関連論文
- Pepsinogen Secretion from Cultured Rat Gastric Mucosal Cells
- Trifluoromethyl Ketones Show Culture Age-dependent Inhibitory Effects on Low K+-induced Apoptosis in Cerebellar Granule Neurons
- Mechanisms of Gastric Mucus Secretion from Cultured Rat Gastric Epithelial Cells Induced by Carbachol, Cholecystokinin Octapeptide, Secretin, and Prostaglandin E2
- Effect of Glucocorticoid on Expression of Rat MUC5ACmRNA in Rat Gastric Mucosa in Vivo and in Vitro
- P2-202 ナジフロキサシンクリーム製剤における先発及び後発医薬品の製剤的比較(一般演題 ポスター発表,後発医薬品,臨床から学び臨床へと還元する医療薬学)
- Mechanisms of Gastric Mucus Secretion from Cultured Rat Gastric Epithelial Cells Induced by Carbachol, Cholecystokinin Octapeptide, Secretin, and Prostaglandin E_2
- Gastric Mucin Secretion from Cultured Rat Epithelial Cells
- Postnatal Changes and effects of glucocorticoid on MUC5AC mRNA expression in the rat stomach.
- Urease inhibitory activity of simple alpha,beta-unsaturated ketones.
- Structural requirements of hydroxylated coumarins for in vitro anti-Helicobacter pylori activity.
- Postnatal changes in ghrelin mRNA expression and in ghrelin-producing cells in the rat stomach
- HETEROCYCLIZATION OF 4-TRIFLUOROACETYL-1,3-OXAZOLIUM-5-OLATES WITH 1,4-BIS-NUCLEOPHILES
- Induction of Apoptosis in Cultured Rat Gastric Epithelial Cells by Ipriflavone : Comparison with Indomethacin (Pharmacology)
- Postnatal Changes and Effects of Glucocorticoid on MUC5AC mRNA Expression in the Rat Stomach
- Effect of Glucocorticoid on Expression of Rat MUC5AC mRNA in Rat Gastric Mucosa in Vivo and in Vitro
- Some Factors Which Influence Intrinsic Factor Content and Its mRNA Level in the Rat Stomach
- Regulation of Gastric Mucosal Pepsinogen and Intrinsic Factor Contents, and Their mRNA Levels during Starvation and Refeeding in Rats
- ALTERATION IN GASTRIC MUCOSAL ACID PROTEASE ACTIVITY INDUCED BY NECROTIZING AGENTS AND PREVENTION BY PROSTAGLANDIN E_2
- EFFECTS OF GASTROINTESTINAL HORMONES AND THEIR RELATED COMPOUNDS ON GASTRIC MOTILITY IN THE RAT
- ROLE OF ADRENERGIC AGONISTS ON GASTRIC SECRETION IN THE RAT
- Interaction among Secretagogues on Pepsinogen Secretion from Rat Gastric Chief Cells
- Secretion of Intrinsic Factor from Cultured Rat Gastric Chief Cells
- Inhibitory Action of Somatostatin on cAMP Dependent Pepsinogen Secretion from Rat Gastric Chief Cells : Involvement of Pertussis Toxin-Sensitive G-Protein
- Somatostatin Inhibits Pepsinogen Secretion without Influencing Cytosolic Free Ca^ Increase Induced by Carbachol and Cholecystokinin Octapeptide in Rat Chief Cells
- Direct Inhibition of Pepsinogen Secretion from Rat Gastric Chief Cells by Somatostatin
- Trifluoromethyl Ketone-Based Inhibitors of Apoptosis in Cerebellar Granule Neurons
- Some Factors Which Influence Intrinsic Factor Content and ItsmRNA Level in the Rat Stomach
- Regulation of Gastric Mucosal Pepsinogen and Intrinsic FactorContents, and Their mRNA Levels during Starvation and Refeeding in Rats
- POSTNEONATAL CHANGES IN GHRELIN mRNA EXPRESSION AND GHRELIN- PRODUCING CELLS IN THE RAT STOMACH
- EFFECT OF GASTRIC SETROGEN ON GHRELIN EXPRESSION AND PRODUCTION IN THE RAT STOMACH