磁気ナノマーカーとSQUIDセンサを用いた磁気的免疫検査システムの開発

概要

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Biological immunoassay is the detection of biological materials called antigen. We have been developing a magnetic-immunoassay system utilizing magnetic marker and high T_c superconducting quantum interference device (SQUID). The magnetic marker was made of Fe_3O_4 nanoparticle with nominal diameter of 25 nm. The particle was coated with polymer, and the antibody was immobilized on the surface. Since the Fe_3O_4 particle with diameter of 25 nm can keep remanence, we used the remanence measurement method for the detection of the marker. The remanent magnetic field from the marker was measured with the highly sensitive SQUID sensor. We conducted an experiment on the detection of the biological materials called IL8 and IgE. At present, a few atto-mol of IL8 and IgE was detected, which shows the high sensitivity of the present method. We also discussed the binding process between the marker and the antigen, and showed the high binding efficiency of the present marker.
九州大学の論文