アフィニティクロマトグラフィーによる^<125>I標識PTHの精製法

概要

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Iodine-125-PTH was purified using affinity gel column chromatography. The column was prepared with activated CH Sepharose 4B coupled to PTH antiserum. Radiommunoassay (RIA) using I-125-PTH (1-34) purified by affinity chromatography with anti-PTH (1-34) Sepharose was sharped than those purified by gel chromatography with Bio Gel P-4. The intra-and inter-assay coefficients of variation were 7.3% (n=5) and 1.5% (n=5), respectively, in the RIA system for PTH (1-34) in human serum. The minimal detectable concentration of PTH(1-34) was 5 pg/tube, and 50 pg/ml in serum was possible to assay. These results indicate that the purification of iodine-125-PTH (1-34) using affinity chromatography with anti-PTH (1-34) Sepharose is a useful method for the establishment of the high sensitive RIA system.
公益社団法人日本放射線技術学会の論文
1987-02-01