Simultaneous Recording of Motile Responses of Light-Absorbing and Reflecting Chromatophores in Vitro(Physiology)

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A method for recording simultaneously the motile responses of both light-absorbing and reflecting chromatophores is described. A microscope in which specimens were observable both by usual transmission and by dark-field incidence illumination systems was put for use. By making use of a rotating photo-chopper disk, the paths of the transmission and the incidence illumination beams were opened alternately at more than 10 Hz. The resultant output currents of the photosensor in two trains, which were endowed with the changes due to activities of above-mentioned two sorts of cells, respectively, were first converted into voltages. They were then appropriately amplified, and led to two separate channels by making use of two sample-and-hold circuits, each of which was triggered synchronously with the opening of the corresponding illuminator path. In each channel, a certain DC component was appropriately subtracted from the output voltage of the sampleand-hold device, and the resultant voltage changes were widely registered on a dual channel electronic chart recorder as a practically smooth trace. Some examples of the application of the present method were presented, and its usability was discussed.
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