ニワトリ脊椎神経節細胞におけるゴルジ装置

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The Golgi apparatus in spinal ganglion cells of the domestic fowl was studied by means of osmium- and silver-impregnation methods, sudan black techniques and, in fresh material, with the phase contrast microscope and the supravital staining. In the osmium or silver-impregnation preparations, the Golgi apparatus appears at the first glance to be a network, composed of black or dark brown strands, in the cytoplasm around the nucleus, Close observations reveal, however, that the strands are not anastmosed, but are independent from each other. Observations of fresh ganglion cells with phase optics indicate the presence of canaliculate or vacuolar structures around the nucleus, which correspond in form and position to the strands in the osmium- or silver-preparations. They also are independent of each other without showing any anastomosis. Most probably, they represent the Golgi apparatus in the fresh cells. The Golgi strands are not stained with sudan black. There are other granules which are distinctly sudanophile. The Golgi strands also are not stained supravitally with neutral red. The other granules, which are homogeneously stained with the dye, correspond to the sudanophile granules and seem to represent the lipochondria.
1960-04-15