フナ肝細胞のゴルジ装置

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The Golgi apparatus in liver cells of silver carp, Carassius auratus, was studied by means of osmium- and silver-impregnation methods, cytochemical techniques and supravital staining methods, and by phase contrast microscopy in fresh material. The Golgi apparatus was found in the osmium- or silver-preparations as several blackened strands and spherules assembled at one side of the nucleus, or scattered along the intracellular bile canaliculus. Observations of fresh liver cells with the phase optics showed clear canaliculate structures which seem to correspond to the strands in the osmium- or silver preparations. The lipochondria lying in most cases near the Golgi area and along the bile canaliculus were observed as small blackened bodies in the osmium- and silver-preparations, but each of them was found to be an elementary myelin figure when carefully examined in the fresh cell by phase microscopy. The Golgi strands and spherules were not stained supravitally with neutral red, although their walls took the dye on prolonged staining. The lipochondria were stained easily. The lipochondria were colored with sudan black, but not the Golgi apparatus. The liver sinusoids responded strongly to alkaline phospatase, while the Golgi elements gave no such reaction. The intracellular bile canaliculus which shows impregnation by the Golgi technique, extends towards, and terminates in, the Golgi area. In some cases, the canaliculi and the Golgi strands or spherules were connected by very fine strands.
社団法人日本動物学会の論文
1959-07-15

フナ肝細胞のゴルジ装置

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