ウニ卵の人工處女生殖の研究 : XVIII.尿素・アルブミン液中における膜形成

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If the sea urchin eggs are immersed in 1 M urea solution for a few seconds and then removed into sea water, activation takes place and the fertilization membrane begins to elevate. But if the eggs are not removed into sea water, the membrane formation does not occur in urea solution. I found that if albumen was added to urea solution, the membrane was formed in this solution. I used 3 species of sea urchins. Stongylocentrotus pulcherrimus was found to be the best material for this experiment. The unfertilized eggs, deprived of egg jelly, are put into urea albumen sotution. The fertilization membrane begins to elevate after 20seconds, and the elevation almost completes in 1 minute. The best concentration of albumen is about 1%. If the eggs are transferred into sea water the membrane elevation has been completed, the formed membrane sinks down on the egg surface. Gelatine, agar agar and arabian gum are ineffective. The eggs are fertilizable after the membrane formation if the eggs are removed into sea water.
社団法人日本動物学会の論文
1950-06-15