ウニ卵の人工處女生殖の研究 : XVII.サポニンによる膜形成の條件

概要

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Saponin is known to be one of the most effective reagents to activate the sea urchin eggs. Some experiments weremade to stuby the effects of concentration, temperature and pH of saponin sea water on the membrane formation. The effects of some ions in the medium ware also studied. The materials used were Heliocidaris crassipina, Pseudocentrotus depressus and Strongylocentrotus pulcherrimus. The interesting results obtained are as follows: 1. In Pseudocentrotus eggs, activation does not occur when temperature is lower than 10℃. In Strongy ocentrotus eggs, activation seldom occurs when temperature is lower than 5℃. 2. Activation is inhibited when pH is lower than 5.5. Cytolysis takes place at pH 2.8. 3. Ca is indispensable to the membrane for mation. When Ca-free sea water is used, the eggs begin to swel' and cytolysis takes place.
社団法人日本動物学会の論文
1950-06-15