放射化分析法によるテトラヒメナのDNA, RNA定量

概要

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1. Commercially available salmon sperm DNA was activated by exposing to a neutron flux of approximately 4×10^<12> n/cm^2/sec for an hour at the pneumatic tube of the reactor of Kyoto University. Immediately after activation, samples emitted β-and γ-rays, due to ^<32>P and ^<24>Na, respectively, but after 7 days' cooling, only β-rays from ^<32>P were detectable. The counts of β-rays then gave the amount of DNA-P. Sensitivity of this method under the conditions described above eas found to be 0.5 μg DNA. 2. Contents of DNA and RNA in Tetrahymena pyriformis W. were determined this method. They were 2.77-3.44×10^<-6> μg-P/cell and 41.9-47.8×10^<-6> μg-P/cell, respectively. In this case, DNA was separated by DNase from nucleprotein and residual proteins were removed by precipitation wigh formic acid. 3. To improve the sensitivity of this activation analysis of nucleic acids in the living cell, it is necessary to minimize the loss of nucleic acids or cells from the small amounts of samples in the process of extraction.
社団法人日本動物学会の論文
1966-06-15