放射化分析法によりATPase活性を定量するための基礎的研究

概要

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Adenosine triphosphatase activity was determined using activation analysis. This method was based on neutron activation of released inorganic phosphates derived from ATP-ATPase enzymic reaction. The neutron activation was carried out by irradiating the paper chromatogram, on which the reaction products were separated, in a pneumatic tube of KUR with a thermal neutron flux of approx. 2.34×10^<13>n・cm^<-2>・sec^<-1> (irradiation time: 20min). An optical density on the autoradiogram was measured with densitometer. The outline of the discussion for the procedure of ATPase assay and results of application for protozoa are as follows. 1) Acetone-formic acid system was a very effective developper for separating the reaction products, viz., ATP, AMP and Pi. Moreover, no radioactivities were induced after neutron activation of the solvent. 2) By using this method, Apyrase activity could be measured with a very high sensitivity, viz., more than 200 fold as compared to ordinary methods. 3) This method was applied for determining Apyrase activity in Tetrahymena pyriformis. The number of cells needed for one assay was about 2.8×10^3.
社団法人日本動物学会の論文
1973-03-15