吉田肉腫における"バルーン細胞"の細胞学的研究I.

概要

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It was found that the supernatant of rat Yoshida sarcoma ascites fluid obtained by ultracentrifugation at 40,000 r.p.m. for 1 hr, still possessed tumor transplantability. Careful examination of the supernatant revealed the presence of unusually giant, so-called "balloon cells". Cells of a similar nature have been reported to occur in the Ehrlich ascites tumors of mice under similar conditions. There were, in general, two or more nuclei located peripherally in a balloon cell. The cytoplasmic materials were generally found localized aroumd the nuclei. The vacuole-like parts of balloon cells were negative for DNA, RNA, protein, polysaccharide, and lipid staining. Repeated inoculations of the supernatant into normal rats resulted in progressiv increases of the number of ballon cells in the ascites fluid of each passage: from 50 per ml. of the supernatant at the first passage to 350,000 at the eighth. The modal chromosome-number of the ascites tumor induced by the supernatant inoculation was constantly 43, in accordance with that of the original one. It is very likely that the tumor formation in rats inoculated with the supernatant may be due to the subsequent proliferation of ballon cells, although other possinle factors or mechanisms for tumor formation can not be excluded at the present status of study.
社団法人日本動物学会の論文
1966-05-15