体外成熟過程におけるマウス卵母細胞と二価陽イオン

概要

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The Effects of calcium and magnesium on follicle cell-free mouse oocytes during in vitro spontaneous maturation were investigated. During maturation, the resistance of the oocytes to a one-hour exposure to medium devoid of divalent cations changed. A gradual increase in the number of oocytes surviving after exposure to the Ca-Mg free medium was observed from the start of germinal vesicle breakdown to the first metaphase of the maturation division. Maturation of oocytes was no longer affected by various concentrations of divalent cations in the medium after the first meiotic metaphase. The rate of calcium uptake by the oocytes increased as maturation proceeded and reached a maximum around the first meiotic metaphase. It then decreased to a level which was higher than that observed before the begenning of maturation. During in vitro maturation, the change in the resistance of mouse oocytes to a brief exposure to a Ca-Mg free medium may indicate that the divalent cations change from a diffusible to a bound state and accumulate within the oocyte until the first meiotic metaphase.
社団法人日本動物学会の論文
1979-09-25