メダカ精巣成分分画の卵割誘導効果について

概要

論文の詳細を見る
Determination of a "cleavage initiating substance" was attempted. Unfertilized eggs of the medaka were injected with testis fractions obtained by centrifugation of homogenate of the testis. Sediment fractions obtained by centrifugation at 100×g for 10min or 10,000×g for 60min induced cleavage. The supernatant fraction at 10,000×g failed to induce cleavage. Electron micrographs of pellets of the sediment fractions showed that the 100×g sediment contained nuclei, mitochondria, both broken and intact sperm heads and sperm tails, and unidentified vesicular and granular bodies. The 10,000×g sediment contained intact or broken sperm tails, and vesicular and granular structures. These results imply that the sperm tails, and/or the vesicular and granular structures present in both the 100×g and 10,000×g sediments participate in initiating cleavage.
社団法人日本動物学会の論文
1973-06-25