Effects of Inhibitors on Forskolin- and Testosterone-Induced Steroid Production by Preovulatory Medaka Follicles

概要

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In follicles of the medaka Oryzias latipes, forskolin（F）stimulated production of both estradiol-17β（E_2） and 17α,20β-dihydroxy-4-pregnen-3-one（17α,20β-diOHP）during 20 hr of incubation, while exogenous testosterone stimulated only the former. Effects of actinomycin D, amanitin, cycloheximide and puromycin on the F and testosterone-induced steroid production were investigated during an in vitro culture system for preovulatory ovarian follicles. When isolated preovulatory follicles were incubated in medium containing actinomycin D, amanitin, cycloheximide or puromycin combined with F or with testosterone, F- and testosterone-induced E_2 and 17α,20β-diOHP production was inhibited. A similar inhibitory effect was also observed with cyanoketone, which is a specific inhibitor of 3β-hydroxy-Δ^5-steroid dehydrogenase（3β-HSD）. These results suggest that the F- and testosterone-induced E_2 and 17α,20β-diOHP production in medaka ovarian follicles is probably mediated by de novo synthesis of mRNA.
愛知教育大学の論文