Modification of Prolactin Receptor (PRL-R) Expression by PRL in the Mouse Liver: Estimation of the Ratio of Two Forms of PRL-R mRNAs by "One-Sided Competitive PCR"
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概要
- 論文の詳細を見る
We developed new means of measuring the ratio of the short to the long form (S/L ratio) of the mouse prolactin receptor (mPRL-R) cDNA by PCR using a primer common to the two forms and two specific primers. A means of estimating the amount of mPRL-R cDNA by competitive PCR was also established. We confirmed that these procedures were valid, since the S/L ratio of standard DNA was unaltered by one-sided cPCR amplification under the following conditions: the ratio was between 0.1 and 4, and the amount of cDNA was between 10^3 and 10^7 molecules/tube. The result of one-sided cPCR showed that the short form was dominant in the mouse liver, while the long form was dominant in other tissues. In addition, pituitary grafting increased the S/L ratio in the liver, implying that prolactin down-regulated the functional long form of PRL-R and lowered tissue sensitivity to prolactin itself by modifying the post-transcriptional regulation of PRL-R.
- 社団法人日本動物学会の論文
- 1996-06-15
著者
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Matsuda Masaru
Laboratory of Reproductive Biology, National Institute for Basic Biology
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Matsuda Masaru
Department Of Aquatic Bioscience Tokyo University Of Fisheries
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Matsuda Manabu
Department Of Biological Science Graduate School Of Science University Of Tokyo
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Mori Takao
Depdrtment of Biological Sciences, Graduate School of Science, University of Tokyo
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Mori Takao
Depdrtment Of Biological Sciences Graduate School Of Science University Of Tokyo
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