Steroidogenesis in the Ovarian Follicles of the Medaka (Oryzias latipes) during Vitellogenesis and Oocyte Maturation
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概要
- 論文の詳細を見る
Changes in the steroidogenic pathway in medaka (Oryzias latipes) ovarian follicles during vitellogenesis and oocyte maturation were investigated in vitro by incubation of follicles with several radiolabeled steroid precursors, followed by thin layer chromatography (TLC) fractionation and recrystallization. When vitellogenic follicles collected at 18 hr before the expected time of spawning were incubated with ^3H-labeled pregnenolone, the major metabolites were 17α-hydroxypregnenolone, 17β-hydroxyprogesterone, and androstenedione. Incubations of vitellogenic follicles with androstenedione produced testosterone and estradiol-17β. By contrast, when maturing follicles (postvitellogenic follicles undergoing maturation) collected at 10 hr before spawning were incubated with ^3H-labeled pregnenolone, the major metabolites were 17α-hydroxypregnenolone, 17α-hydroxyprogesterone, and 17α, 20β-dihydroxy-4-pregnen-3-one (17α, 20β-DP, maturation-inducing hormone of medaka); androstenedione was not detected. Neither vitellogenic and maturing follicles produced progesterone when they were incubated with ^3H-labeled pregnenolone, suggesting that in medaka ovarian follicles both estradiol-17β and 17α, 20β-DP are synthesized by the ^5Δ-steroid pathway. Thus, there is a distinct shift in the steroidogenic pathway from estradiol-17β to 17α, 20β-DP production in medaka ovarian follicles, and it is suggested that the decrease in C17, 20-lyase activity is responsible for this shift. The phosphodiesterase inhibitor IBMX enhanced androstenedione production in incubations of vitellogenic follicles with ^<14>C-labeled progesterone. Calcium ionophore A23187 and the phorbol ester TPA (a protein kinase C activator) blocked the stimulatory actions of IBMX on androstenedione production. These findings suggest that multiple signalling pathways may participate in the regulation of ovarian steroidogenesis, and further emphasize the importance of calcium as a regulator of P-450c17 activity.
- 社団法人日本動物学会の論文
- 1996-12-15
著者
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KOBAYASHI Daisuke
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Josai University
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TANAKA MINORU
Laboratory of Breeding Science, Graduate School of Fisheries Sciences, Hokkaido University
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Tagawa M
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
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Kobayashi Daisuke
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Nagahama Yoshitaka
Department of Molecular Biomechanics, The Graduate University for Advanced Studies
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Tanaka M
Kyoto Univ. Kyoto Jpn
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Fukada Sachiko
Laboratory Of Reproductive Biology National Institute For Basic Biology
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Tanaka M
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
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Tanaka M
Tokyo Inst. Technol.
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Nagahama Yoshitaka
Department Of Developmental Biology National Institute For Basic Biology
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Kobayashi Daisuke
Department Of Applied Chemistry Faculty Of Science And Technology Keio University
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Tanaka Minoru
Laboratory Of Breeding Science Graduate School Of Fisheries Sciences Hokkaido University
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Kobayashi D
Kanazawa Univ. Ishikawa
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Nagahama Yoshitaka
Department of Clinical Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University
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