IN SITU HYBRIDIZATION HISTOCHEMISTRY WITH OLIGODEOXYNUCLEOTIDE PROBE AS A TOOL FOR THE DETECTION OF NEUROPEPTIDE mRNAs (<Symposium>In situ Hybridization I. : Methodology)
スポンサーリンク
概要
- 論文の詳細を見る
In situ hybridization histochemistry with synthetic oligodeoxynucleotide probes was applied to investigate the localization of tissue mRNAs. Specific probes for mRNAs to vasopressin- and oxytocin-preprohormone were designed by using the computer-based homology search program. The probes were labeled with ^3H-deoxycytidime triphosphate (dCTP) or ^<55>S-deoxyadenosine triphosphate (dATP) by terminal deoxynucleotidyl transferase. The specificity of the tailed probe was again confirmed by the homology search. Issues for the properties of isotopes and data analysis of the hybridizatioin signals in the autoradiography were discussed from the methodological point of view.
- 日本組織細胞化学会の論文
著者
-
Kawata Mitsuhiro
Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Japan
-
YURI Kazunari
Department of Anatomy, Kochi Medical College
-
Kawata Mitsuhiro
Department Of Anatomy And Neurobiology Graduate School Of Medical Science Kyoto Prefectural Universi
-
Yuri Kazunari
Department Of Anatomy Kochi Medical School
-
Yuri Kazunari
Department Of Anatomy Kyoto Prefectural University Of Medicine
-
Kumamoto Kenzo
Department Of Anatomy Meiji University Of Oriental Medicine
-
Kumamoto Kenzo
Department Of Anatomy Faculty Of Acupuncture Kyoto Meiji University Of Oriental Medicine
-
Kumamoto Kenzo
Department Of Anatomy Meiji College Of Oriental Medicine
-
Kawata Mitsuhiro
Department Of Anatomy Kyoto Prefectural University Of Medicine
関連論文
- Aldosterone-Sensitive Nucleus Tractus Solitarius Neurons Regulate Sensitivity of the Baroreceptor Reflex in High Sodium–Loaded Rats
- Colocalization of mineralocorticoid receptor and glucocorticoid receptor in the hippocampus and hypothalamus
- Estrogen reduces the neurite growth of serotonergic cells expressing estrogen receptors
- Sex Hormone Receptors and Brain Cell Structure
- P2-06 GFP imaging analysis of subcellular localization of androgen receptor and estrogen receptor in a single living cell
- Visualization of effects of estrogen to the brain by using GFP
- Intracellular dynamics of steroid hormone receptor
- 2 Aortic root replacement : indications and long-term results in various aortic root pathologies(Symposium 4 (SY-04) (H) Recent Progress in the Treatment of Aortic Diseases,Special Program,The 72nd Annual Scientific Meeting of the Japanese Circulation Soc
- The distributions of apoptotic cells in the medial preoptic areas of male and female neonatal rats
- Localization of Estrogen Receptors α and β in the Articular Surface of the Rat Femur
- Appearance of LFA-1 in the initial stage of synaptogenesis of developing hippocampal neurons
- EXPRESSION OF THE GLUCOCORTICOID RECEPTOR IN RAT IS INVOLVED IN CHANGES IN PROOPIOMELANOCORTIN PROCESSSING PATTERNS
- Expression of Prostaglandin E Receptor EP_4 Subtype in Rat Adrenal Zona Glomerulosa: Involvement in Aldosterone Release
- EXPRESSION AND LOCALIZATION OF THE PUTATIVE MEMBRANE PROGESTERONE RECEPTOR 25-DX IN THE DEVELOPING PURKINJE CELL(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Distribution of P300 and SRC-1 in the Rat Brain
- Real-Time Imaging of Corticosteroid Receptor Dynamics in Living Cells with a Green Fluorescent Protein and its Spectral Variants
- II-C-05 Immunohistochemical study on the postnatal development of glucocorticoid receptor (GR), mineralocorticoid receptor (MR), SRC-1 and p300 in the rat brain(THE 45TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- Subcellular and Subnuclear Distributions of Estrogen Receptor α in Living Cells Using Green Fluorescent Protein and Immunohistochemistry
- Subcellular distribution of estrogen receptor α visualized with green fluorescent protein
- Tetrasomy 12 in ovarian tumors of thecoma-fibroma group : A fluorescence in situ hybridization analysis using paraffin sections
- IMPROVEMENT OF TECHNIQUE OF IMMUNOHISTOCHEMICAL DEMONSTRATION OF BIOACTIVE SUBSTANCES IN THE CENTRAL NERVOUS SYSTEM
- Central Nervous System of the Reeler Mutant Mouse: A Golgi and Fluorescent Histochemical Analysis
- Distribution of NADPH-Diaphorase-Positive Neurons in the Mouse Brain : Differences from Previous Findings in the Rat Brain and Comparison with the Distribution of Serotonergic Neurons
- Changes in Nitric Oxide Synthas-Containing Neurons in the Brain of Thiamine-Deficient Mice
- IN SITU HYBRIDIZATION HISTOCHEMISTRY WITH OLIGODEOXYNUCLEOTIDE PROBE AS A TOOL FOR THE DETECTION OF NEUROPEPTIDE mRNAs (In situ Hybridization I. : Methodology)
- Direct and Efficient Cytosolic Delivery Using Oligoarginine Vectors in the Presence of Pyrenebutyrate
- Counteranion-Mediated Delivery of Arginine-Rich Peptides into Living Cells
- Distribution of glucocorticoid receptor immunoreactivity and mRNA in the rat brain: an immunohistochemical and in situ hybridization study
- Estrogen and Non-Feminizing Estrogen for Alzheimer's Disease
- Distribution of BNP containing neurons in the mammalian spinal cord
- Brain Sex and Stress Response by Molecular Dynamics of Receptor Proteins(Recent Progress and Perspective in Steroid Research,Symposium,PROCEEDING OF THE 75^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
- Uptake of Exogenous Caerulein in the Brain of the Rat : A Immunohistochemical Study
- Study of vibrissal follicle sensory endings using confocal laser scanning and electron microscopy.
- Nerve Fibers Originating from the Brain in the Rat Pineal Complex
- IIB-18 Three-dimensional analysis of the innervation pattern of touch domes in skin folds of cat paws(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- Postnatal Development of NADPH-Diaphorase Activity in the Rat: The Role of Nitric Oxide in the Ontogeny of Arginine Vasopression and Oxytocin
- Postnatal development of NADPH-diaphorase activity in the rat hypothalamo-hypophysial axis with special reference to vasopression and oxytocin
- REAL-TIME IMAGING OF CORTICOSTEROID RECEPTORS IN LIVING CELLS
- P-33 Real-time imaging of green fluorescent protein-tagged human β2-adrenergic receptor in living cells
- In vitro and in vivo immunocytochemistry for the distribution of mineralocorticoid receptor with the use of specific antibody
- S1-2 Visualization of Glucocorticoid Receptor and Mineralocorticoid Receptor Interactions in Living Cells with GFP-based Fluorescence Resonance Energy Transfer (FRET)(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- Subcellular distribution of lipocortin 1(LC1)in the folliculostellate(FS)cells of rat anterior pituitary following adrenalectomy and corticosterone treatment : A possibility of the role for intercellular communication in the anterior pituitary
- PI-44 Direct synaptic inputs from a gastrin-releasing peptide system to neurons of the spinal nucleus of bulbocavernosus revealed by the HVEM
- A MODIFIED ALUMINUM-FORMALDEHYDE (ALFA) HISTOFLUORESCENCE METHOD FOR ENHANCED VISUALIZATION OF CENTRAL CATECHOLAMINE
- Aldosterone-Sensitive Nucleus Tractus Solitarius Neurons Regulate Sensitivity of the Baroreceptor Reflex in High Sodium-Loaded Rats
- Vacuum-assisted closure with Safetac technology for mediastinitis in patients with a ventricular assist device
- Distribution of estrogen receptor βmRNA-containing cells in ovariectomized and estrogen-treated female rat brain
- Estrogen receptor-immunoreactive neurons contain calcitonin gene-related peptide, methionine-enkephalin or tyrosine hydroxylase in the female rat preoptic area
- Immunohistochemical Analysis of Gomori-Positive Fibers in the External Layer of the Rat Median Eminence
- The Distribution of Steroid Hormone Receptors in The Spinal Cord
- Glucocorticoid and Apoptosis in the Hippocampus (第4回産業医科大学国際ワークショップ : 情動・ストレス・代謝)
- S2-5 In Situ Hybridization Histochemistry as a Tool for the Elucidatioin of Structure and Function in the Brain
- Identification of mRNAs of Oxytocin, Vaso-pressin and Tyrosine Hydroxylase by In Situ Hybridization with Oligonucleotide Probe
- Roles of steroid hormones and their receptors in structural organization in the nervous system
- Negative pressure wound therapy for left ventricular assist device-related mediastinitis : two case reports
- Successful treatment of mediastinitis after ventricular assist device implantation with rerouting of the outflow vascular prosthesis
- How many hair follicles are innervated by one afferent axon? A confocal microscopic analysis of palisade endings in the auricular skin of thy1-YFP transgenic mouse