THE PROCEDURE FOR ULTRACYTOCHEMICAL DEMONSTRATION OF DEHYDROGENASES IN THE RAT ADRENAL CORTEX USING COPPER FERROCYANIDE
スポンサーリンク
概要
- 論文の詳細を見る
This is a procedure for the ultracytochemical demonstration of 3β-hydroxy-steroid dehydrogenase (3β-HSD) and glucose-6-phosphate dehydrogenase (G6PD) and the localization of these enzymes in the adrenocortical cell of rat is presented. The procedure involves pre-fixation of tissues, tissue sectioning and incubation of specimens. Brief fixation (for 30min) in a mixture of glutaraldehyde and formaldehyde (0.25%: 1% and 2%: 2%) or 2.7% glutaraldehyde was excellent to preserve both the activity of 3β-HSD and G6PD and fine cellular structure. Unfrozen sections obtained by Vibratome (Oxford) were superior to frozen sections obtained by a cryostat for preservation of the ultrastructure and enzyme activity of the cell. Sections used were 40-100μm in thickness. In the present method dehydroepiandrosterone (DHA), etio-cholane-3β-17-one (etiocholane) and pregnenolone were utilized as substrate, potassium ferricyanide as a final electron acceptor instead of tetrazolium salt, and phenazine methosulfate (PMS) as an intermediate electron carrier. As a result, the activity of 3β-HSD was localized in the cytoplasmic matrix and intracristal space of mitochondria. The activity of G6PD was visualized mainly in the cytoplasmic matrix near the plasma membrane. The reaction did not take place in any cells incubated either in the substrate-free medium or in the medium markedly inhibited by respiratory chain inhibitors such as Rotenone and Antimycin A. The findings mentioned so far were evidently those of the specific ultracytochemical reaction. Several problems will be discussed in this paper concerning the procedure for the ultracytochemical demonstration.
- 日本組織細胞化学会の論文
著者
-
SHIGEMATSU Kazuto
Department of Pharmacology 2
-
Kawai Kioko
Dept. Of Pathology Nagasaki University
-
Kawai Kioko
Department Of Pathology 2 Nagasaki University
-
Kawai Kioko
Department Of Pathology 2 Nagasaki University School Of Medicine
-
Tsuchiyama Hideo
Department Of Pathology 2 Nagasaki University
-
Shigematsu Kazuto
Department Of Pathology 2 Nagasaki University
-
Shigematsu Kazuto
Department Of Pharmacology 2:department Of Pathology 2 Nagasaki University School Of Medicine
関連論文
- GASTRIC SUBMUCOSAL HETEROTOPIA OF THE IMMATURE GASTRIC GLANDS WITH ENDOSCOPIC FEATURE MIMICKING CARCINOID TUMOR
- SUCCESSFUL ENDOSCOPIC BAND LIGATION FOR TREATMENT OF POSTPOLYPECTOMY HEMORRHAGE
- Systemic Effects of Carteolol, a β-Adrenoceptor Antagonist in Stroke-Prone Spontaneously Hypertensive Rats
- Angiotensin II and Alpha-human Atrial Natriuretic Polypeptide Binding Sites in the Human Adrenal Gland
- Changes and recoveries of endocrine-vascular environments of the hypothalamus and the pituitary glands induced by long-standing vigorous smoking three-dimensional analysis of hormone producing cells and capillaries by confocal laser scanning microscopy
- Confocal Laser Scanning Microscopic Imaging of Subcellular Organelles, mRNA, Protein Products, and the Microvessel Environment
- B-8 Three-dimensional (3D) imaging of the hormone-secreting cells and their microvessels environments in estrogen-induced prolactinoma of the rat pituitary gland by confocal laser scanning microscopy (CLSM)
- Three Dimensional Imaging of Tumor Angiogenesis in DMBA Induced Rat Mammary Cancer by Confocal Laser Scanning Microscopy(CLSM).
- Three-Dimensional (3D) Imaging of Tumor Angiogenesis and its Inhibition : Evaluation of Tumor Vascular-Targeting Agent Efficacy in the DMBA-induced Rat Breast Cancer Model by Confocal Laser Scanning Microscopy (CLSM)
- PROLIFERATING CELL NUCLEAR ANTIGEN IN ACTH-INDEPENDENT BILATERAL MACRONODULAR ADRENAL HYPERPLASIA
- HISTOCHEMICAL AND ULTRASTRUCTURAL OBSERVATION OF THE PIGMENT IN PIGMENTED LIPID HISTIOCYTES OF CHRONIC GRANULOMATOUS DISEASE
- Histochemical and Ultrastructural Observation of the Pigment in Pigmented Lipid Histiocytes (PLH) of Chronic Granulomatous Disease (CGD)
- A Pathology Study of Malignant and Benign Ovarian Tumors Among Atomic-Bomb Survivors : Case Series Report
- Combined adrenal adenoma and myelolipoma in a patient with Cushing's syndrome : Case report and review of the literature
- Enzyme-histochemical Study in Early Lesions and Adenocarcinomas Induced in Rat Small Intestine by ENNG
- Adrenocorticotropic hormone-independent bilateral macronodular adrenocortical hyperplasia associated with Cushing's syndrome
- Histochemical observation on experimentally induced carcinoma in the glandular stomach of rats. (No.2)
- ULTRACYTOCHEMICAL LOCALIZATION OF DEHYDROGENASE IN THE RAT ADRENAL CORTEX
- Histochemical Study of the Human Adrenocortical Pigments
- Histochemical Study of the Cortical Adenoma and Non-tumorous Adrenal Tissue in Primary Aldosteronism
- HISTOCHEMICAL OBSERVATIONS ON NODULAR PORTIONS IN ADRENAL CORTEX OF AGING RATS
- AN ELECTRON MICROSCOPICAL STUDY OF THE ADRENAL MEDULLA IN SPONTANEOUSLY HYPERTENSIVE RATS, PARTICULARLY ON CATECHOLAMINE-GRANULES IN SECRETING CELLS
- Histochemical Study in Nodular Portions of the Adrenal Cortex in Old Rats
- Histochemical Study on the Effect of Aldactone-A Treatment for the Adrenal Cortex
- HISTOCHEMICAL DISTRIBUTION OF THE SECONDARY ALCOHOL DEHYDROGENASE IN THE RAT, ESPECIALLY IN THE ADRENAL CORTEX
- Localization of Secondary Alcohol Dehydrogenase in the Rat Adrenal Cortex after Administration with SU 4885 (Metopirone)
- Histochemical and morphological characteristics in culture of normal human adrenal cortex and adrenocortical tumors
- Histochemical Study of Endocrine Cells in Adenocarcinomas of the Glandular Stomach and Small Intestine of Rats induced by Carcinogens
- Comparative Studies of Corticosteroid Content between Non-functioning Adenomatous Hyperplasia and Functioning Adenoma
- Effects of Potassium Loading and Other Stimulating Conditions on the Glomerular Zone of Fetal Adrenal Cortex of Rats
- Electron Microscopic Study of Adrenal Cortex in Spontaneously Hypertensive Rat
- Partial Characterization of IGF-I in Primary Human Lung cancers
- THE PROCEDURE FOR ULTRACYTOCHEMICAL DEMONSTRATION OF DEHYDROGENASES IN THE RAT ADRENAL CORTEX USING COPPER FERROCYANIDE
- Histoautoradiographical and Electron Micro-Scopical Studies of the Prenatal Adrenal Medulla in Spontaneously Hypertensive Rat
- Role of Connective Tissue Growth Factor and Angiotensin II in Tubulointerstitial Fibrosis in Experimental Obstructive Nephropathy
- The Spontaneously Hypertensive Rat (8) : Proceedings of VIII Annual Scientific Meeting of the Council for the Spontaneously Hypertensive Rat October 8, 1972, Nagasaki
- COMPARATIVE STUDIES BETWEEN HORMONE CONTENTS AND MORPHOLOGICAL APPEARANCES IN HUMAN ADRENAL CORTEX
- The Histochemical and Ultrastructural Changes of the Adrenal Cortex in SHR Given NaCl Solution
- Effects of MPC-1304, a Novel Calcium Antagonist, on Stroke-Prone Spontaneously Hypertensive Rats
- Expression of the Endothelin Receptors in the Hippocampus of SHRSP after Transient Forebrain Ischemia
- Evaluation of the neuroprotective action of WEB 1881 FU on hypoglycemia/hypoxia-induced neuronal damage using rat striatal slices.
- Morphological Study on the Thyroid Gland in SHR Treated with Thyroxine
- Localization and Characterization of Specific 125I-Brain Natriuretic Peptide (26) Binding Sites in Adrenal Gland and Kidney
- Cell Proliferation and Cell Differentiation of the Adrenal Medulla in Prenatal Spontaneously Hypertensive Rats
- Cell Proliferation in the Adrenal Cortex of the Fetus of Spontaneously Hypertensive Rat Injected with Tritiated Thymidine
- Antihypertensive effects of CD-349, a novel 1, 4-dihydropyridine derivative on stroke-prone spontaneously hypertensive rats
- Morphological Study of the Adrenal Cortex in Stroke-Prone Spontaneously Hypertensive Rats (SHRSP)
- Endothelin (ET) receptor binding sites and expression of ET mRNAs in the rat lower brainstem
- Immunohistochemical and Morphometrical Studies of the Pituitary Gland in SHR