Ultrastructural localization of glial cell line-derived neurotrophic factor (GDNF) and its receptor RET in rat spinal dorsal horn
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概要
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GDNF, a neurotrophic factor, is thought to promote the regeneration and survival of injured motor and sensory neurons in the peripheral nervous system. It is generally believed that GDNF is target-derived and is retrogradely transported in these neurons. However, recent reports have suggested that a subgroup of dorsal root ganglion (DRG) neurons synthesize GDNF themselves and anterogradely transport the GDNF towards spinal dorsal horn (SDH). In the present study, we examined the anterograde transport of GDNF immunohistochemically and observed GNDF immunoreactive products (GDNF-IR) in the cisterna of the rough endoplasmic reticulum of DRG neurons using electron microscopy. The anterogradely transported GDNF in the SDH co-localized with substance P(SP) and/or CGRP within dense-core vesicles located in the axon terminals. These findings suggest the possibility that the GDNF may act in conjunction with SP and/or CGRP in the SDH following nociceptive stimulation. Furthermore, the distribution of RET, a GDNF receptor, was examined by immuno-electron microscopic methods in the SDH of animals following rhizotomy, hemisection of spinal cord, or colchicine treatment. RET immunoreactivity in the SDH was ultrastructurally localized in both astroglia and nerve terminals originating from neurons within the spinal cord. Thus, the GDNF that is transported from the DRG is thought to influence the secondary sensory neurons and astroglia of the SDH.
- 日本組織細胞化学会の論文
著者
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Ohta Keisuke
Department Of Anatomy & Histology Kurume University School Of Medicine
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Ohta Keisuke
Department Of Applied Biological Science Frontier Research Center For Genomic Drug Discovery Tokyo U
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