High-yield Secretion of the Recombinant Sweet-Tasting Protein Thaumatin I
スポンサーリンク
概要
- 論文の詳細を見る
Thaumatin, an intensely sweet-tasting protein used as a sweetener, was secreted by the methylotrophic yeast Pichia pastoris. Approximately 100 mg L-1 of recombinant thaumatin I was obtained using an expression vector which possesses three copies of the thaumatin gene containing the 22-amino acid pre-sequence. Expression yield was about three-fold higher than when the α-factor secretion signal from Saccharomyces cerevisiae was used. The circular dichroism and tryptophan fluorescence spectra for recombinant thaumatin I were almost the same as those for plant thaumatin. Large amounts of homogeneous recombinant thaumatin allowed for preparation of high-quality crystals in the presence of cryoprotective glycerol used in high-resolution x-ray structural analysis to help further understand the perception of the sweet taste of thaumatin.
- 社団法人 日本食品科学工学会の論文
- 2010-11-01
著者
-
Ide Nobuyuki
Department Of Cancer Biology Dana-farber Cancer Institute Harvard Medical School
-
Ohta Keisuke
Department Of Anatomy & Histology Kurume University School Of Medicine
-
Masuda Tetsuya
Department Of Biological Science And Technology The University Of Tokushima Graduate School
-
Kitabatake Naofumi
Department Of Natural Resources Graduate School Of Global Environmental Studies Kyoto University
-
Ohta Keisuke
Department Of Natural Resources Graduate School Of Global Environmental Studies Kyoto University
-
Masuda Tetsuya
Department Of Natural Resources Graduate School Of Global Environmental Studies Kyoto University
-
Ide Nobuyuki
Department Of Natural Resources Graduate School Of Global Environmental Studies Kyoto University
-
Masuda Tetsuya
Department Of Animal Science And Resources College Of Bioresource Sciences Nihon University
関連論文
- Glycosylphosphatidyl inositol-anchored protein (GPI-80) gene expression is correlated with human thymoma stage
- Effect of ethanol on mouse hepatitis virus-induced cytotoxicity
- Hepatocellular Carcinoma in Keio Affiliated Hospitals : Diagnosis, Treatment, and Prognosis of this Disease
- Kupffer cell-mediated cytotoxicity against hepatoma cells occurs through production of nitric oxide and adhesion via ICAM-1/CD18
- An Ergosterol Peroxide, a Natural Product That Selectively Enhances the Inhibitory Effect of Linoleic Acid on DNA Polymerase β
- Galactosyldiacylglycerol, a Mammalian DNA Polymerase α-Specific Inhibitor from a Sea Alga, Petalonia bingbamiae
- Action of a New Mammalian DNA Polymerase Inhibitor, Sulfoquinovosyldiacylglycerol
- Sulfoquinovosyldiacylglycerol, KM043,a New Potent Inhibitor of Eukaryotic DNA Polymerases and HIV-Reverse Transcriptase Type 1 from a Marine Red Alga, Gigartina tenella
- Anti-tumor Effect of Chemically Synthesized Sulfolipids Based on Sea Urchin's Natural Sulfonoquinovosylmonoacylglycerols
- Downregulation of Tie2 gene by a novel antitumor sulfolipid, 3'-sulfoquinovosyl-1'-monoacylglycerol, targeting angiogenesis
- Structure-Activity Relationship of a Novel Group of Mammalian DNA Polymerase Inhibitors, Synthetic Sulfoquinovosylacylglycerols
- Propofol pharmacokinetics in a patient with bilateral leg amputation
- Tracheal intubation by paramedics under limited indication criteria may improve the short-term outcome of out-of-hospital cardiac arrests with noncardiac origin
- Thermoelectric Properties of Ca-Mg-Si Alloys
- High-yield Secretion of the Recombinant Sweet-Tasting Protein Thaumatin I
- A-24 LM and EM Cytochemistry of 5'-Nucleotidase in the Superficial Dorsal Horn of the Rat Spinal Cord
- Subtilisin-like Proprotein Convertase PACE4 is Required for Skeletal Muscle Differentiation
- I-B-11 Localization of Monocarboxylate Transporters MCT1 and MCT4 in Rat Skeletal Muscle (2^ report)(THE 45TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- B-16 A morphological investigation of the relationship between arachnoid membrane and the core of arachnoid granulations by 5^1-nucleotidase histochemistry
- Combined Effect of Pre-Straining and Pre-Aging on Bake-Hardening Behavior of an Al-0.6mass%Mg-1.0mass%Si Alloy
- In reply : Is out-of-hospital intubation by paramedics valid enough to be continued?
- Evaluation of Heat Deterioration of Hard Milk Fat as a Function of Pressurization Time by Chemiluminescence Analysis
- Ultrastructural localization of glial cell line-derived neurotrophic factor (GDNF) and its receptor RET in rat spinal dorsal horn
- Regional Diminution of von Willebrand Factor Expression on the Endothelial Covering Arachnoid Granulations of Human, Monkey and Dog Brain.
- Development of a PCR/LDR/Flow-Through Hybridization Assay Using a Capillary Tube, Probe DNA-Immobilized Magnetic Beads and Chemiluminescence Detection