Antisense oligodeoxyribonucleotide targeting cAMP response element-binding protein inhibits growth of rat submandibular gland in vitro
スポンサーリンク
概要
- 論文の詳細を見る
The transcription factor, cyclic AMP response element-binding protein (CREB), occurs in the nuclei of acinar cells of the rat submandibular gland during the early postnatal periods. To elucidate the role of CREB in the growth of submandibular gland, the effect of an antisense oligodeoxyribonucleotide (ODN) targeting CREB was examined in the organ culture system of neonatal rat submandibular gland. In the presence of antisense-CREB ODN in the culture media for 48 hr, a significant decrease in the growth of cultured gland was observed in terms of both the area of acinar epithelial tissue and 3H-thymidine-labeling index of acinar cells, with concomitant decrease in the nuclear immunoreactivity for CREB without any degenerative changes in the histology of the gland. In contrast, sense-CREB ODN exhibited no effect on acinar growth. Administration of the β-adrenergic agonist isoproterenol (IPR) induced enhanced proliferation of acinar cells, which was also inhibited by antisense-CREB ODN. In contrast, the proliferation of connective tissue cells was neither enhanced by IPR nor inhibited by antisense-CREB ODN. These results suggest that the transcription factor CREB plays specific roles in the proliferation of submandibular gland acinar cells during the early postnatal periods.
- 日本組織細胞化学会の論文
著者
-
Iseki Shoichi
金沢大学 医研究 第1解剖
-
Iseki Shoichi
Department Of Histology And Embryology Graduate School Of Medical Science Kanazawa University
-
Iseki Shoichi
Department Of Anatomy Kanazawa University School Of Medicine
-
Amano Osamu
Department of Histology and Embryology, Graduate School of Medical Science, Kanazawa University
-
Amano O
Kanazawa Univ.
-
Amano Osamu
Department Of Histology And Embryology Graduate School Of Medical Science Kanazawa University:depart
-
井関 尚一
Department of Anatomy, School of Medicine, Kanazawa Univeristy
関連論文
- Involvement of carnitine/organic cation transporter OCTN2 (SLC22A5) in distribution of its substrate carnitine to the heart
- A time- and cost-saving method of producing rat polyclonal antibodies
- IIA-09 The cost- and time-saving method of producing polyclonal antibodies(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- Visualization of the Specific Gene Transcription in the Nucleus with a Novel In Situ RNase Protection Method
- Urinary Trypsin Inhibitor : Production in the Liver and Reabsorption in the Kidney of the Rat
- P-21 Expression and Localization of Urinary Trypsin Inhibitor in the Rat Embryo
- Coexpression of menin and JunD during the duct cell differentiation in mouse submandibular gland
- IIB-20 A novel protein from mouse submandibular gland whose expression is downregulated by androgens(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- Expression of the Adhesion Molecule Spermatogenic Immunoglobulin Superfamily (SgIGSF) in Mouse Tissues.
- Role of the spermatogenic-Sertoli cell interaction through cell adhesion molecule-1 (CADM1) in spermatogenesis
- Expression and localization of the cell adhesion molecule SgIGSF during regeneration of the olfactory epithelium in mice
- Effects of Heavy Oil from the Nakhodka on Hepatic and Pulmonary Enzymes of Mice(PROCEEDINGS OF 24TH SYMPOSIUM ON TOXICOLOGY AND ENVIRONMENTAL HEALTH)
- Immunohistochemical localization of vascular endothelial growth factor in the endocrine glands of the rat
- 30-II-07 Expression of the transcription factor JunD in the diffferentiation of the duct system of mouse submandibular gland(THE JOINT MEETING OF THE 44TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY AND THE 35TH ANNUAL MEETING
- The role of cyclic AMP response element-binding protein in the differentiation of granular convoluted tubule cells in the rat submandibular gland
- In situ hybridization detection of the mRNA for growth factors and their receptors in the rodent organs
- Antisense oligodeoxyribonucleotide targeting cAMP response element-binding protein inhibits growth of rat submandibular gland in vitro
- II-B-17 Co-expression of menin with JunD in the duct system of mouse submandibular gland(THE 45TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- A-9 Immunohistochemical localization of Vascular endothelial growth factor (VEGF) in the endocrine glands of the rat
- Insulin-like growth factors, hepatocyte growth factor and transforming growth factor-α in mouse tongue myogenesis
- Co-expression of NGF and Its High-affinity Receptor TrkA in the Rat Carotid Body Chief Cells
- PDZK1 Regulates Organic Anion Transporting Polypeptide Oatpla in Mouse Small Intestine
- Compensatory upregulation of myelin protein zero-like 2 expression in spermatogenic cells in cell adhesion molecule-1-deficient mice