IMMUNOHISTOCHEMICAL LOCALIZATION OF Na^+, K^+-ATPase IN HUMAN LIVER
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概要
- 論文の詳細を見る
The localization of Na^+, K~+-ATPasc in human liver was investigated on the light and electron microscopic levels by using anti-sera on the catalytic subunit of Na^+, K~+-ATPase in human kidney. α-subunit of Na^+, K^+-ATPase was extracted from the gel of SDS-PAGE as an immune antigen and injected into rabbits in order to produce the antibody. Under light microscopic examination, reaction products were recognized at the bile canalicular, lateral and sinusoidal membranes of hepatocytes and luminal membrane of biliary epithelial cells on the PLP (periodate-lysine-paraformaldehyde) fixed frozen sections. By ultrastructural observation, reaction products were also recognized at the bile canalicular membrane of hepatocytes and luminal membrane of biliary epithelial cells. These results suggest that Na^+, K^+-ATPasc is responsible for bile formation and transport processes on these membranes.
- 日本組織細胞化学会の論文
著者
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HARA Toru
Department of Physics, Faculty of Science, Okayama University
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ASANO Goro
Department of Pathology, Nippon Medical School
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Asano Goro
Department Of Pathology Nippon Medical School
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Hara Toru
Department Of Pathology Nippon Medical School
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Hayashi Tomohiro
Department Of Pathology Nippon Medical School
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Hayashi Tomohiro
Department Of Chemistry Faculty Of Engineering Gunma University
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Asano G
Central Inst. Electron Microscopic Res. Tokyo Jpn
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Hara Toru
Department Of Materials Science And Engineering Teikyo University
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Hayashi Tomayoshi
Department Of Pathology Nagasaki University Hospital Nagasaki University Graduate School Of Biomedic
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KUDOU MITSUHIRO
Department of Pathology, Nippon Medical School
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OTAKE KIYOKO
Department of Pathology, Nippon Medical School
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Asano Goro
The Department Of Pathology Nippon Medical School
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Otake Kiyoko
Department Of Pathology Nippon Medical School
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Hayashi Tomayoshi
Department Of Pathology Nippon Medical School
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Kudou Mitsuhiro
Department Of Pathology Nippon Medical School
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