Nitric Oxide Production and Energy State in the Heart after Endotoxin Administration

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To evaluate nitric oxide(NO)production and the energy state of the heart after endotoxin administration, Wistar rats were injected ip with 10 mg/kg Escherichia coli lipopolysaccharide(endotoxin). Morphologic changes, plasma nitrite concentration, expression of inducible NO synthase(iNOS), and cardiac energy state, as reflected by several metabolites, were observed chronologically 0(control), 4, 6 and 8h after endotoxin administration. Electrocardiography(ECG)demonstrated arrhythmia after endotoxin administration. Biochemically, NO production increased in blood and iNOS increased in the heart. The amount of myocardial β-ATP measured by ^<31>P magnetic resonance spectroscopy(^<31>P-MRS)increased transiently and then decreased. This transient increase might be a hyperdynamic response to endotoxin administration. At 4 and 6h after endotoxin administration, pH measured by ^<31>P-MRS was slightly decreased, but this decline was not statistically significant. On the other hand, the amount of lactate in heart samples increased in the ^1H magnetic resonance spectra(^1H-MRS). Ultrastructurally, in cardiovascular tissue, intracytoplasmic organelles were observed to be injured in blood vessels and cardiomyocytes associated with mast cell infiltration. These results suggest significant metabolic and morphologic abnormalities in the heart after endotoxin administration.
社団法人日本循環器学会の論文
1998-06-20