HISTOCHEMICAL LOCALIZATION OF ALKALINE PHOSPHATASE ACTIVITY IN PERITUBULAR ZONE OF POSTNATAL TESTES IN RATS

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The histochemical localization of nonspecific alkaline phosphatase activity (ALPase) on the limiting membrane of seminiferous tubules was investigated in the testes from the rats aged 1, 7, 14, 21, and 42 days. On thick (ca 40 μ) sections incubated in Burstone's medium for ALPase, a cribriform distribution (about 36 -45 μ in width) of the strong activity began to be detected on 14 days of age and it was established by 21 days postnatal. Closing about the basement membrane of seminiferous tubules, an inner cellular layer of the flattened cells showing many of the characteristics of smooth muscles began to be demonstrated ultrastructurally on 14 days of age due to the differentiation of the outer cellular layer. At that time, moderate activity of ALPase began to be seen throughout a myoid cell layer by Ogawa et al.'s ultracytochemical method. Abundant reaction products for ALPase could be proved more than 21 days of age. Many pinocytotic vesicles within the cytoplasm of the myoid cells were filled with final products, and the strong activity was also observed in the space surrounding the myoid cells. Finally, the peritubular zone appeared virtually adult in ultrastructure and in both localization and activity of ALPase by 21 days postnatal. By electrophoresis on starch gel, the isozyme pattern of ALPase demonstrated by azo dye method was studied on the testicular tissues at the same stages of postnatal development as design of the histochemical investigation. Type II of the isozyme increased moderately more than 14 days after birth. A cribriform distribution of ALPase on the wall of seminiferous tubules might be related to the activity surrounding the myoid cell, which would be Type II of ALPase.
日本組織細胞化学会の論文

HISTOCHEMICAL LOCALIZATION OF ALKALINE PHOSPHATASE ACTIVITY IN PERITUBULAR ZONE OF POSTNATAL TESTES IN RATS

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