Endocytosis in the Epithelial Cells of the Endolymphatic Sac
スポンサーリンク
概要
- 論文の詳細を見る
To investigate the properties of endocytosis of the epithelial cells in the intermediate portion of the endolymphatic sac (ES), we infused cationized ferritin (CF), microperoxidase (MPO), and horseradish peroxidase (HRP), as endocytosis tracers, into the endolymphatic space of the ES. Thirty minutes after infusion, the tissues were fixed and the distributions of HRP, MOP and CF were observed by transmission electron microscopy. The results at 30 min after tracer infusion were as follows. Concerning CF, endocytosis showed different activity depending on the area of the ES. In one area, CF was hardly observed within the epithelial cells, while in another area, it was observed within the epithelial cells. The CF-loaded vesicles were mainly coated vesicles. These results suggested that infusion of the artificial endolymphatic sac endolymph with CF may not stimulate the whole signal system of the ES. Concerning HRP, it bound to the apical membrane and was observed in the vesicles and the sorting endosomes. The diameters of these vesicles were of two types : one had a smaller diameter (less than 200 nm), while the other had a larger diameter (over 200 nm) and originated from macropinocytosis. Concerning MPO, it was much less observed in the vesicles than HRP. We discuss the different activities of endocytosis among these tracers, and suggest that a large molecular weight substance, i. e. HRP, might stimulate the fluid-phase endocytosis and/or adsorptive pinocytosis.
- 日本組織細胞化学会の論文
著者
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AZUMA HIROSHI
Department of Botany, Graduate School of Science, Kyoto University
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瀬口 春道
高知大学 医学部 機能形態学教室
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OKADA Teruhiko
Department of Anatomy, Kochi Medical School
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Seguchi Harumichi
Dept. Anat Cell Biol Kochi Med Sch
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Seguchi H.
Department Of Anatomy And Cell Biology Kochi Medical School
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SEGUCHI Harumichi
department of Cell Biology, Kochi Medical School
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Sawada Shoichi
Department of Otolaryngology, Kochi Medical School
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Takeda Taizo
Department of Otolaryngology, Kochi Medical School
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Kakigi Akinobu
Department of Otolaryngology, Kochi Medical School
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Takeuchi Shunji
Department of Otolaryngology, Kochi Medical School
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Higashiyama Kasumi
Department of Otolaryngology, Kochi Medical School
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Yamakawa Kazuhiro
Department of Otolaryngology, Kochi Medical School
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Yamakawa Kazuhiro
Laboratory For Neurogenetics Riken Brain Science Institute
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Yamakawa Kazuhiro
Department Of Otolaryngology Kochi Medical School
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Okada Teruhiko
Department Of Anatomy And Cell Biology Kochi University Faculty Of Medicine
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Takeda T
Department Of Otolaryngology Kochi Medical School
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Takeda Taizo
Department Of Otolaryngology Kouchi Medical School
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Sawada Shoichi
Department Of Otolaryngology Kochi Medical School
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Kakigi Akinobu
Department Of Otolaryngology Kochi Medical School
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Takeuchi Shunji
Department Of Otolaryngology Kochi Medical School
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Azuma Hiroshi
Department Of Biosystem Regulation Institute Of Biomaterials And Bioengineering
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Seguchi Harumichi
Department Of Anatomy And Cell Biology Kochi Medical School
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Higashiyama Kasumi
Department Of Otolaryngology Kochi Medical School
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Azuma Hiroshi
Department Of Otolaryngology Kochi Medical School
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Okada Teruhiko
Dep. Of Anatomy And Cell Biology Kochi Univ. Fac. Of Medicine
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Okada Teruhiko
Department Of Anatomy And Cell Biology Kochi Medical School
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YAMAKAWA Kazuhiro
Department of Applied Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-sen 11, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
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