組織培養法による泌尿器悪性腫瘍のfibrinolysisの研究

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In order to estimate fibrinolytic activity produced by human urologic malignant cells, surgical specimens from the malignant and normal tissues have been studied by a monolayer culture method. The fibrinolytic activity yielded into the supernatant medium and fibrin degradation products (FDP) resulted from coagulum lysis present in the system were determined by using a fibrin plate method and FDP assay Kit (Wellcome Reagents Ltd.), respectively, The localization of fibrinolytic activity in the cultivated cells was studied by a histochemical fibrin slide technique with a slight modification. The results obtained were as follows: 1. An active growth of epithelial cells was observed in 11 renal cell carcinomas, a Wilms' tumor, an angioleiomyolipoma, 2 transitional cell carcinomas of the renal pelvis, and 4 transitional cell carcinomas of the bladder. 2. In the case of normal tissues, the active growth was observed in the cortex of 17 kidneys and the medulla of 4 kidneys. No active growth was observed in mucous membranes from a renal pelvis and 5 bladders. 3. Using the fibrin plate method, no fibrinolytic activity was detected in supernates from the renal cell carcinomas, the transitional cell carcinomas of the bladder, and HeLa cells.
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