膀胱癌患者の細胞性免疫能に関する研究 : 第4報自家および他家膀胱癌粗抽出液を用いたLAI, LMI testについて

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The specific reactivities of lymphocytes to tumor associated antigens were studied on 19 patients with bladder carcinoma and 12 age matched control subjects. The specific reactivities were measured by means of leukocyte adherence inhibition test (LAI) and leukocyte migration inhibition test (LMI). Autologous and allogeneic crude extracts were prepared from resected bladder carcinoma tissue for antigen. Tumor and normal tissues were homogenized and centrifuged at 20,OOOg, 4℃ for 60 minutes. Supernate filtered by millipore filter (0.45μ) was used as antigen. Final concentration of tissue extracts was adjusted to 100μg/ml of protein. LAI test was done according to the method of Halliday and LMI test was according to Mcillmurray.
社団法人日本泌尿器科学会の論文