耐性緑膿菌の R プラスミドの分離と耐性伝達に関する研究

概要

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At two hospitals where gentamicin (abbreviated as GM) resistant strains of Pseudomonas aeruginosa had been isolated with high incidences, organisms of Pseudomoms aeruginosa which are resistant to amimglycosides were collected in order to isolate their R plasmids carrying resistance to aminoglycosides including GM and examine their genetic properties. Furthermore, each of these organisms was phage typed and analyzed for their relationship to the source of GM resistant R plasmids. A total of 141 strains of P. aeruginosa, consisting or 41 strains isolated at hospital A and 100 strains isolated at hospital B, were examined. Using the agar plate dilution method, strains having resistance to GM, dibekacin (DKB), tobramycin (TOB) and amikacin (AMK) were selected and used as the donor organism for an experiment on transconjugants. For preparing transconjugants, two methods were applied : one was to transfer resistance in a broth conjugally and the other on a membrane filter. As the recipient organism for conjugated transference, P. aeruginosa, ML 4262 (trp-his-ilv-met- rif^r) and ML 4600 (trp-his-) were used.
社団法人日本泌尿器科学会の論文