Spermatogenesisに関する研究 : 第2編 : 器官培養下におけるspermatogenesisに対するtestosteroneとFSHの作用機序の検討 : 睾丸機能の研究 : 第8報

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The effect of glucose, testosterone and FSH on spermatogenesis was studied in organ cultures of 21 day-old rat testis. Since previous experiments on the determination of appropriate experimental conditions for rat testicular culture suggested that the best maintenance of primary spermatocytes was 2 mg/ml of glucose, 50 ug/ml of testosterone and 200 μg/ml of FSH, the present investigation was undertaken to define the actions of these additives in the aforementioned concentrations. Data from 7 days and 14 days of cultures were statistically analyzed by analysis of variance. As the interpretation of the mode of action of these factors on spermatogenesis is very intricte, data obtained in this series of experiments may be summarized by opinion. The results are summarized as follows. 1) In cultures of 2 mg/ml of glucose medium containing a concentration of glucose double that normally present in a control medium, it was demonstrated that differentiation of germ cells was not induced by addition of glucose, while good maintenance of primary spermatocytes was observed for at least 7 days in culture. This suggests that glucose, as the energy source, acts on Sertoli cells to give nutrients to germ cells. 2) In cultures of 50 μg/ml concentration of testosterone medium, it was demonstrated that the number of resting, leptotene and zygotene spermatocytes, i.e. early stages of primary spermatocytes, which have been associated with differentiation of spermatogonia increased. A reduction in the number of pachytene spermatocytes, a late stage of primary spermatocytes, and an increase in the number of pyknotic cells were noticiable, since the supplying capacity of Sertoli cells could not be accompanied by an increase in the number of early stages of primary spermatocytes (an explanation is given in subsection 3, concerned with the action of FSH).
社団法人日本泌尿器科学会の論文
1978-12-20

Spermatogenesisに関する研究 : 第2編 : 器官培養下におけるspermatogenesisに対するtestosteroneとFSHの作用機序の検討 : 睾丸機能の研究 : 第8報

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