Spermatogenesisに関する研究 : 第1編 : 器官培養下におけるspermatogenesisに対するtestosteroneとFSHの基礎的検討 : 睾丸機能の研究 : 第7報

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The effect of glucose, testosterone and FSH on spermatogenesis was studied in organ cultures of 21-day-old rat testes by modification of Trowell's method. The control medium used in all cultures consisted of MEM (Eagle's minimum essential medium), supplemented with 10% fetal calf serum, 1 mM of sodium pyruvate, 0.1 mM each of alanine, aspartic acid, asparagine, glutamic acid, glycine, proline and serine, and 3 mM of glutamine and penicillin 100 U, per ml. The culture medium was maintained at PH 7.1 and explants were incubated at 31℃ in a gas atmosphere of 5% CO_2 and 95% air. The results are summarized as follows. 1) In the control medium, primary spermatocytes showed a marked dimution in number during 4 to 7 days in culture, and gradually disappeared in almost all tubules within 14 days in culture, even though spermatogonia were well maintained and mitotic activity continued after 42 days in culture. 2) Sertoli cells in all tubules were well maintained at an almost definite number of 16 to 19 per seminiferous tubules for as long as 42 days. 3) Effects of glucose : In cultures grown in 2 mg/ml concentration of glucose medium, the number of primary spermatocytes was slightly greater than in the control medium containing 1 mg/ml of glucose for 7 days in culture. In cultures grown in a 3 mg/ml concentration of glucose medium, however, the seminiferous tubules showed moderate diminution in diameter and moderate thickening of the tubular wall with the number of primary spermatocytes being smaller than in the control medium.
社団法人日本泌尿器科学会の論文
1978-12-20

Spermatogenesisに関する研究 : 第1編 : 器官培養下におけるspermatogenesisに対するtestosteroneとFSHの基礎的検討 : 睾丸機能の研究 : 第7報

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