Bacillus属細菌由来菌体外酵素の解析と利用

概要

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Many species of the genus Bacillus produce a variety of extracellular enzymes, some of which are of industrial importance. Studies on these extracellular enzymes are significant not only from a practical standpoint, but also a theoretical point of view of better understanding the secretion mechanism and structure-function relationship of enzymes. We have established a host-vector system of Bacillus subtilis with many kinds of plasmid vectors with different copy numbers. Low copy numberplasmids such are pTB53 and pTB522 (copy number about 8) for B.subtilis were extremely useful. We have cloned and analyzed many extracellular enzyme genes such as proteases, amylases, and penicillinase. Gene cloning contributed for increase of productivity of target enzymes and moreover cloned genes can be used for further studies about enzyme gene expression regulation and strategic improvement of enzyme characteristics through protein engineering. In this report, giving the cases of neutral protease from Bacillus stearothermophilus and penicillinase from Bacillus licheniformis as examples, four subjects, (1) Cloning and sequencing of structural genes, (2) Analyses of gene expression regulation, (3) Strategic alteration of thermostability of extracellular enzymes and a regulatory protein, and (4) Use of regulatory region for extracellular expression of foreign genes will be introduced and discussed.
社団法人日本生物工学会の論文
1995-03-25