4,5-ジヒドロキシクマリンによるベリウムの蛍光定量

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The 4, 5-dihydroxycoumarin with acid dissociation constants of K_<a1>=2.72×10^<-3> and K_<a2>=2.88×10^<-14> is able to form complexes with 22 metal ions. Among these metal ions, 12 could form fluorescent complexes. The analytical procedure for the spectrofluorometric determination of Be was as follows : To a solution containing 0.1〜1.3 μg of Be, 2.5 ml of 2×10^<-4> M 4, 5-dihydroxycoumarin in methanol, 10 ml of methanol, and a sufficient amount of 0.5 M ammonia solution to adjust the pH of 9〜10 were added, and this mixture was diluted to a final volume of 25 ml with water. After standing for 1 h, the fluorescence intensity was measured at 428 nm with excitation at 334 nm. The calibration curve was linear in the range of 0.1〜1.3 μg Be, and the relative standard deviation obtained from seven measurements of 0.9 μg Be was 0.73%. Cr (III), Ti (IV), Mn (II) and La (III) gave serious negative errors. The molar ratio of Be to the reagent was 1 : 1 at pH 6.0 and 1 : 2 at pH 8.5〜11.5.
社団法人日本分析化学会の論文
1988-06-05