MICRO DETERMINATION OF TOBRAMYCIN IN SERUM BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
スポンサーリンク
概要
- 論文の詳細を見る
A rapid, simple, and accurate method for the determination of tobramycin in serum by high-performance liquid chromatography (HPLC) has been developed. The method is sensitive to 0.2 *g/ml using only 20 *l of serum. The serum proteins are precipitated with methanol. To the supernatant, a counter-ion reagent is added, then an aliquot of the solution is injected into the chromatograph. The determination of tobramycin is performed by a combination of reverse-phase, ion-pair chromatography, post-column derivatization with o-phthalaldehyde, and fluorescence detection. Comparisons with a microbiological assay and with a homogeneous enzyme immunoassay gave a correlation coefficient of 0.941 and that of 0.978, respectively.
- 社団法人日本分析化学会の論文
- 1982-08-05
著者
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KUBO Hiroaki
School of Pharmaceutical Sciences, Kitasato University
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KINOSHITA Toshio
School of Pharmaceutical Sciences, Kitasato University
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Kobayashi Yoshie
School Of Pharmaceutical Sciences Kitasato University
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Kinoshita Toshio
School Of Pharmaceutical Sciences Kitasato University
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NISHIKAWA Takashi
School of Medicine, Kitasato University
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Kubo Hiroaki
School Of Pharmaceutical Sciences Kitasato University
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Nishikawa Takashi
School Of Medicine Kitasato University
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