FLUOROPHOTOMETRIC ENZYME IMMUNOASSAY OF THYROXINE IN DRIED BLOOD SAMPLES ON FILTER PAPER

概要

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We report a sensitive fluorophotometric enzyme immunoassay (EIA) for measuring thyroxine (T_4) in dried blood samples on filter paper. Anti-T_4 IgG (rabbit) and the T_4-horseradish peroxidase conjugate of the Enzymun Test T_4 (Boehringer) were used. Bound and free fractions of the enzyme conjugate were separated by the antibody solid phase method, using anti-T_4 IgG-coated bead. The enzyme activity of the bound fraction was determined fluorophotometrically at excitation and emission wavelengths of 320 and 405 run, respectively, using 3-(p-hydroxyphenyl)-propionic acid and hydrogen peroxide as substrate. Standard curves between 1.7 and 22 *g T_4/dl were prepared from T_4 standards of dried blood spots. The intra-and inter-assay coefficients were 4.9 - 13.7% and 13.7 - 19.5%,respectively. The present EIA method was used in preliminary mass-screening tests for T_4.
社団法人日本分析化学会の論文
1982-02-05