RADIOIMMUNOASSAY OF CYCLAZOCINE AND STEREOSPECIFICITY OF ANTIBODY
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概要
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A new radioimmunoassay, using ^3H-cyclazocine, rabbit antiserum and charcoaldexttan separation of bound and free cyclazocine, for the direct analysis of serum cyclazocine is described. This method, which is specific for cyclazocine and has a detection limit of ca. 25 pg/assay tube, was successful in determining the cyclazocine level in the sera of beagles intramuscularly injected with 3 or 10μg/kg cyclazocine. The drug half-life was 90 min ; the apparent distribution volumes were 4.0 and 5.26 liter/kg, respectively. One of the antisera raised in rabbits immunized with dl-cyclazocine derivative-BSA conjugates was highly specific for l-cyclazocine.
- 公益社団法人日本薬学会の論文
著者
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Tsuji Akio
School Of Pharmaceutical Sciences Showa University
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Maeda Masako
School Of Pharmaceutical Science Showa University
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