ウレアーゼを用いる微量尿素の反射吸光光度定量

概要

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Sample solution (10 μl) was mixed with the urease solution (5 μl), which contained 1800 units urease and 25 ml glycerin in 100 ml, in the tip of a micro pipette. After standing for 20 min, the mixture (15 μl) was dropped on the pH test paper, and the reflection absorbance (λ=570 nm) of the test paper was measured by spectrophotometry. The pH test paper was prepared by placing 40 μl of 0.1% (w/v) Bromophenol Red |pH 5.2 (yellow)〜pH 7.0 (red)| aq. solution (pH 5.2) onto the filter paper (5B) and drying it in a desicator for 12h. A linear relation was obtained between the concentration of urea (5〜120 ngN/10 μl) and the reflection absorbance of the test paper. The detection limit (S/N=3) was 2 ngN/10 μl. Reproducibility tests (n=7) showed that the relative standard deviation of the response was 5.5% for 30 ngN/10 μl.
社団法人日本分析化学会の論文
1996-05-05