細菌性アイノリナーゼ試験法の研究 : (II)酵素学的術技の簡易化ならびに結論

概要

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Simplification of Fujita's method in the bacterial thiaminase test was attempted. Removement of bacterial cells from culture fluids may be abbreviated. Conditions of the reaction were unified in 2/75M phosphate buffer at pH 7.0,40℃ for 60 minutes. However, the optimum conditions should be adopted in the case of assay for each thiaminase activity. Pyridine (10^<-4>M, final concentration) was added in the reaction system in order to enhance the activity of the thiaminase I. It showed neither the inhibition for the activity of thiaminase II nor the disturbance for the estimation of thiochrome. Diastase digestion for the check of phosphorylation of thiamine can be omitted. The simplified method or the direct method in the previous paper was employed for the assay of remaining thiamine after the reaction. Thus, it was demonstrated that the simplified method of bacterial thiaminase test was employed as the substituted method for the Fujita's method because of its convenience and equal sensitivity.
日本ビタミン学会の論文
1967-11-25