ビタミンB_1要求性大腸菌変異株にかんする研究 : ビタミンB_1またはコハク酸を要求する変異株のコハク酸代謝

概要

論文の詳細を見る
A mutant strain, Escherichia coli W2-3,which required either thiamine or succinate for aerobic growth was isolated. Anaerobically the mutant grew on glucose-ammonium salts medium as well as the wild type. The amounts of thiamine required for a half-maximum growth of the mutant were observed to be at least 10 times more than that required by another thiamine-less mutants, whose metabolically blocked steps involved in thiamine biosynthesis had been determined. No experimental evidence, however, could be obtained in this mutant to suggest that the pathway to thiamine was blocked genetically. It seems likely that the reductive pathway to succinate is active under anaerobic but not under aerobic conditions and the addition of external thiamine restores the aerobic formation of succinate from α-ketoglutarate.
日本ビタミン学会の論文
1969-01-25