β-カロチン立体異性体の分別定量と食品中における分布

概要

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Fractional determination of the stereoisomers of β-carotene described by Bickoff was tested and the improved procedure was described. Using hydrated lime as an absorbent and p-cresyl methyl ether in petroleum benzine as a developing solvent mixture, β-carotene was fractionated into three stereoisomers, i. e., neo-β-carotene B(B), neo-β-carotene U (U) and all-trans-β-carotene (T). Since α-carotene appears in the fraction of B, it must preliminarily be separated from β-carotene fraction by the first lime chromatography using petroleum benzine as a developing solvent. The ratio of the isomers was not affected by the duration of saponification. The distribution of the stereoisomers of β-carotene in a number of foodstuffs used for carotene source was determined and given in a table. In each case, T was 80-9O%, whereas each of B and U was below 10%. No other isomers were found. The ratios of the isomers in some fresh tissues were about the same as those of the dehydrated ones.
日本ビタミン学会の論文