重層法による Amylase の測定 (第1報) : 玉川法の検討

概要

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TAMAGAWA's one dimentional method of amylase assay was modified and applied to estimate the enzymic power of diastase and pancreatin in the pharmacopeia and of Taka-diastase. The result obtained by the improved method has satisfied the MASUYAMA's formula.The warm solution at pH 5.0 containing 1% refined agar and 0.05% soluble starch was poured into a glass-tube (8mm × 10 cm) which had been filled at the bottom with absorbent cotton, and the tube was kept cold. To the tube 1 cc of the amylase solution and a few drops of toluol were added and it was incubated at 50℃. After a certain time, the solution was poured off, and a capillary tube was inserted into the column of agar-starch. N/20〜N/50 I-IK solution was puured and the capillary tube was removed. A blue colour of iodine-starch reaction takes place around the agar-starch column.Squared value of the length from meniscus to blue band is proportional to the logarithm of enzyme concentration, so that it enables us to estimate the amylase concentration by measuring the length mentioned.
社団法人日本生物工学会の論文
1951-12-15