微生物による有機化合物の変換反応 : 6,8-Dihydroxypurineの生成反応について

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It was found that the bacteriological transformation of 6,8-dihydroxypurine from purine nucleosides or purine bases, using some species of genuses Serratia, Flavobacterium, or Achromobacter. 6,8-Dihydroxypurine resulted in a compound which is considered as a intermediate metabolic product of purine derivatives such as hypoxanthine, niosine, adenine, and adenosine.The optimum reaction conditions of 6,8-dihydroxypurine formation using Serratia marsescents IFO 3046 were studied, and the following results were obtained.1) 2,6,8-Trihydroxypurine (uric acid) was the only product formed, when the substrate contained the hydroxy or amino group in the 2-position of purine base, but when there was no substitutional group, 6,8-dihydroxypurine could be accumulated.2) Addition of Cu^<++> or Cu^+ to the reaction system was found to be effective for the formation of 6,8-dihydroxypurine.3) Optimum pH of reaction was 7 to 8.It was considered that there are two pathways in the oxidation of purine bases by microorganisms of genus Serratia, Fluvobacterium, or Achromobacter, The one is catalyzed by xanthine oxidase, and the other is though 6,8-dihydroxypurine.
公益社団法人日本生物工学会の論文
1969-04-25