酵母細胞壁の溶解について : (第2報)酵素の精製

概要

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An enzyme which is lytic system against yeast cell walls was obtained from the fungus, Rhizopus sp, and it was purified by chromatography, using on DEAE-Sephadex and CM-cellulose.The β-1,3-glucanase and β-1,6-glucanase were separated from each other by CM-cellulese chromatography.The β-1,3-glucanase hydrolyzed laminarin to glucose as the end product by a random mechanism.The β-1,6-glucanase hydrolyzed luteose almost completely to producing gentiobiose.The purified enzyme showed, lytic activities, against both aceton-dried and heat-treated yeast cells.Both β-1,3-glucanase and protease were not lytic by themselves against the yeast cells harvested in the logarithmic phase and treated with cysteine but the they were lytic against these yeast cells when in case each of them was combined with β-1,6-glucanase.
公益社団法人日本生物工学会の論文
1968-11-25