酵母細胞壁の溶解について : (第1報)菌株の分離と粗酵素について

概要

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The present paper describs on the hydrolysis of yeast cell walls by a lytic enzyme of Rhizopus sp. isolated from soil.This lytic enzyme was highly lytic against both aceton-dried heat-treated cells of Saccharomyces cerevisiae.Yeast cells harvested in the logarithmic phase were slightly hydrolyzed by the enzyme, but in the stationary phase, yeast cells were not hydrolyzed by it at all.Mercaptoethanol facilitated the lysis by this enzymes.When cell suspensions of yeast, harvested in the logarithmic phase and treated with mercaptoethanol, were incubated with this lytic enzyme in the presence of 0.5 M sodium chloride, protoplasts were formed.Isolated cell walls from Saccharomyces cerevisiae were hydrolyzed by the lytic enzymes.
公益社団法人日本生物工学会の論文
1968-11-25