邪魔板なしのジャーファーメンターに関する研究

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Specially designed equipment is called for in studying the bioengineering aspects of submerged fermentation processes involving fungi and streptomyces. The authors designed a non-baffled jar fermentor. The agitator is a high speed oscillating mixer that provides pulsed flow pattern, and angular oscillation is given for agitating the liquid by a delta blade. No baffles are required because of a lack of swirling flows developed by mixing blades.Using a single Orifice, air is introduced into the fermentor.We examined the effects of various operating conditions of the fermentor on the basis of the sulphite oxidation procedure used by Cooper et al. Height, length, and direction of the single or multiblades were varied. Higher aeration efficiencies may be obtained by use of multi-blades type.The non-Newtonian fluid tested was a colloidal suspension, methylcellulose in various concentrations. We determined some rheological characteristics from the flow curves using a Ferranti rotating coaxial cylinder viscometer. The quantitative relation governing the mixing time in non-Newtonian fluid was studied experimentally by using hydrochloric acid and sodium hydroxide as a tracer. A dimensionless equation has been developed which expresses mixing time number (f_t) as a function of a modified Reynolds number (N'_<Re>) and scale ratios. At N'_<Re> more than 5×10^3,f_t became independent of N'_<Re> and the equation.And also we tried an application of our data to the "mixing time factor" (φ) presented by Norwood et al. It would be possible to define a region of laminar for mixing pseudoplastic fluids in such a way. We established the power number-Reynolds number correlation to pseudoplastic fluids with varying flow behavior indexes in our fermentor.Using this fermentor, we got over the difficulties of filamentous fungi or streptomyces cultures in which their rheological properties differ very much from Newtonian.
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邪魔板なしのジャーファーメンターに関する研究

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