大腸菌のトリプトファン蓄積における細胞制御理論の応用 : (第2報) 変異株を使用したトリプトファン蓄積における培地条件の検討

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A previous report showed that the double mutant R^-X_3^-accumulate tryptophan and its accumulation is stimulated by addition of casamino acid and anthranilic acid.In this report, a suitable medium composition has been examined for maximum tryptophan accumulation by mutant R^-X^-_3. To use vitamin B_1 and casamino acid togather was efefctive as a growtih factor, as was also bacto peptone. Glucose was the most suitable carbon source compared with glycerine, and sucrose. Ammonium choloride was the most suitable nitrogen source compared with urea, and ammonium sulfate. Using the medium composition : vitamin B_1,0.01mg/l, anthranic aicd 0.5g/l casamino acid 4g/l, glucose 50g/l, NH_4Cl 3g/l, KH_2PO_4 3g/l, K_2HPO_4 7g/l, MgSO_47aq 0.2g/l, 700μg/ml tryptophan accumulated in medium.
公益社団法人日本生物工学会の論文
1965-05-25