大腸菌のトリプトファン蓄積における細胞制御理論の応用 : (第1報) トリプトファンを蓄積する変異株について

概要

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The purpose of this report is to select the strain that accumulats tryptopha, using the genetic technique. Regulator gene mutants (R^-) and feed back mutants (X_3^-) were selected from Escherichia coli HfrH. A double mutant, the regular gene and the feed back site mutated, R^-X_3^-was also found by using recombination and transduction techniques. Tryptophan acumulation was tested in heterogenote and in these mutants. The heterogenote which carries the transducing particle φ80dt_1 was inhibited in growth by addition of anthranilic acid and did not accumulate tryptophan. Mutants R^- and X_3^-were unable to accumlate tryptopha. Only the double mutant R-X_3^-accumulated 400μg/ml tryptophan in medium. This stratin was able to accumulate tryptophan in basal medium which was composed of in organic salts, glucose and vitamin B_1,and its accumulation was stimulated by the addition of anthranilic acid and casamino acid.
公益社団法人日本生物工学会の論文
1965-05-25