大腸菌のトリプトファン合成系における酵素の制御機構について : (第2報)紫外線照射をうけたheterogenoteにおけるトリプトファン合成酵素の生合成について

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Tryptophan synthetase activity was assayed after UV irradiation of Escherichia coli wild strain 3110,lysogenic strain 3110 (80)^+, and two different heterogenotes.In the wild strain, 3110 and the lysogenic strain 3110 (80)^+, decrease in tryptophan synthetase activity occured from UV irradiation. When the firt heterogenote arising from φ80 dt_1,was treated by UV light, a nonrepressible tryptohan synthetase synthesis was observed before lysis the of cell. But in the second heterogenote arising from transducing particle φ80 dt_0,the same phenomenon did not occur.The reason for these observations may be that the transducing particle φ80 dt_1,carries the tryptophan operon lacking in the anthranilic acid locus, whereas the transducing particle φ80 dt_0 carries a complete operon which does contain an anthranilic acid locus. When the anthranilic acid locus is absent in the operon, the synthesis of tryptophan synthetase is non-repressible during the process of vegetative phage development from prophage after UV-irradiation. These support suggertions in previous paper that a gene concerned with the repression system of tryptophan synthetase is present in the anthranilic acid locus or closely linked to this locus.
社団法人日本生物工学会の論文
1965-04-25