大腸菌のトリプトファン合成系における酵素の制御機構について : (第1報)ファージ感染系におけるトリプトファン合成酵素の生合成について

概要

論文の詳細を見る
The biosynthesis of tryptophan synthetase by Escherichia coli, after infecting with φ80 HFT lysate was studied. The transducing particles φ80 dt present in the HFT lysate incorporate various lengths of the tryptophan operon. The first type of transducing particle, φ80 dt_0,is able to transduce all tryptophan-requiring mutants implying that all the structural genes of the tryptophan operon are incorporated into the phage genome. When the HFT lysate containing this kind of transducing particle infects the A2 tryptophan auxotroph, the synthesis of tryptophan synthetase is repressed by the presence of tryptophan.The second type of transducing particles, φ80 dt_1,and φ80 dt_2,can not transduce anthranilic acid mutants. These transducing particles do not possess the gene involved in the synthesis of anthranilic acid synthetase. When the HFT lysate containing the second type of transducing particle infects the A2 tryptophan auxotroph, the synthesis of tryptopha synthetase is not repressed even in the presence of tryptophan in the medium.These observations suggest that a gene concerned with the regulation of tryptophan synthetase is associated with the anthranilic acid synthetase locus or closely linked to this locus.
社団法人日本生物工学会の論文
1965-04-25