大腸菌のトリプトファン合成系における酵素の制御機構について : (第3報)Heterogenoteにおけるトリプトファン合成系酵素の調節について

概要

論文の詳細を見る
The wild strain 3110 and heterogenotes were grown in a synthetic medium with excess tryptohan. These strains were then washed and regrown for one hour in the same fresh medium containing tryptophan at various concentrations. After centrifugation and washing, the crude extract of the bacterial suspensions were assayed for the A protein and the B protein of tryptophan synthetase and anthranilic acid synthetase.One heterogenote carries the transducing particle, which is unable to synthesise anthranilic acid, is represented by the symbol (A^-B^+ Anth^+/ex A^+B^+ Anth^<del>). In this heterogenote, anthranilic acid synthetase is repressed completely by tryptophan and the B protein of tryptophan synthetase is repressed only partially by tryptophan, but the A protein is not represed at all by tryptophan. The second heterogenote which carries the transducing particle φ80dt_0 is represented by the symbol (A^-B^+ Anth^+/ex A^+B^+ Anth^+.)In this heterogenote, the synthesis of proteins A and B were completely repressed in a manner similar to that of the wild strain.The first heterogenote does not possess the anthranilic acid locus at the side of exogenote and which is similar to the lactose constitutive mutant O^c (Jacob and Monod) in the synthesis of tryptophan synthetase. That is, a change in one locus simultaneously affects the synthesis of A and B protein of tryptophan synthetase. This change affects only the genes in cis position but not trans position because it does not affect the synthesis of anthranilic acid synthetase.From these observations, the operator gene should be included in the anthranilic acid locus or closely linked to the anthranilic acid locus.
社団法人日本生物工学会の論文
1965-04-25

大腸菌のトリプトファン合成系における酵素の制御機構について : (第3報)Heterogenoteにおけるトリプトファン合成系酵素の調節について

スポンサーリンク

概要

著者

関連論文

スポンサーリンク